Rabbit anti-Cytoskeletal Actin Antibody, Affinity Purified A300-485A from Bethyl Laboratories, Inc.

Product Specs
ItemRabbit anti-Cytoskeletal Actin Antibody, Affinity Purified
CompanyBethyl Laboratories, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA300-485A
Quantity100 µg
ApplicationsWB, IHC
Concentration1000 µg/ml
Conjugate/TagUnconjugated
FormatWhole IgG
ReactivityHuman, Mouse
Targetbeta Actin
HostRabbit
Antibody TypePrimary
NCBI Full Gene Nameactin gamma 1
NCBI Gene AliasesACT, HEL-176, DFNA26, DFNA20, ACTG
ClonalityPolyclonal
IsotypeWhole IgG
Domain/Region/TerminusN-terminus
Storage2 - 8°C

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Bethyl Laboratories, Inc.

P.O. Box 850

Montgomery, Texas 77356

United States

Phone: 1-800-338-9579
Fax: 1-866-597-6105

Email: orders@fortislife.com

Company Profile

Website: fortislife.com

(1) Severe acute respiratory syndrome coronavirus 2 infection leads to Tau pathological signature in neuronsPNAS NexusSeptember 1, 2023Di Primio C, Quaranta P, Mignanelli M, Siano G, Bimbati M, Scarlatti A, Piazza CR, Spezia PG, Perrera P, Basolo F, et al.anti-histone H2B, 1:1,000 (sc-515808, Santa Cruz Biotechnology, Dallas, TX, USA); rabbit anti-actin, 1:5,000 (A300-485A, Bethyl Laboratories); and HRP-conjugated anti-Myc-Tag (9B11) (#2040 Cell Signaling Technology). Secondary

(2) circCsnk1g3- and circAnkib1-regulated interferon responses in sarcoma promote tumorigenesis by shaping the immune microenvironmentNature communicationsNovember 25, 2022Piras R, Ko EY, Barrett C, De Simone M, Lin X, Broz MT, Tessaro FHG, Castillo-Martin M, Cordon-Cardo C, Goodridge HS, et al.overnight at 4 °C with anti-MDA5 (#5321, Cell Signaling), anti-RIG-I (#3743, Cell Signaling), anti-β actin (A300-485A, Bethyl), anti-TBK (#3504, Cell Signaling), anti-pTBK (#5483, Cell Signaling), or anti-IRF1 (#8478,

(3) Single-cell RNA-seq of a soft-tissue sarcoma model reveals the critical role of tumor-expressed MIF in shaping macrophage heterogeneityCell ReportsJune 21, 2022Fernando H.G. Tessaro, Emily Y. Ko, Marco De Simone, Roberta Piras, Marina T. Broz, Helen S. Goodridge, Bonnie Balzer, Stephen L. Shiao, Jlenia GuarnerioAFS98Cat: BE0213 Anti-MIF,Cell Signaling Technology,Clone E7T1WCat: 87501 Anti-beta actin,Bethyl,Cat: A300-485A TotalSeq-A0301 anti-mouse Hashtag 1 Antibody 10ug,Biolegend,Cat#155801; RRID: AB_2750032 TotalSeq-A0302

(4) Low-dose self-amplifying mRNA COVID-19 vaccine drives strong protective immunity in non-human primates against SARS-CoV-2 infectionNature communicationsJune 7, 2022Rappaport AR, Hong SJ, Scallan CD, Gitlin L, Akoopie A, Boucher GR, Egorova M, Espinosa JA, Fidanza M, Kachura MA, et al.antibody clone 1A9, GeneTex, Cat#GTX632604 (1:1000). Rabbit Anti-Mouse Actin polyclonal, Bethyl Labs, Cat#A300-485A (1:1000). Goat Anti-Mouse HRP, Bethyl Labs, Cat#A90-116P (1:10,000). Goat anti-Rabbit HRP, Bethyl Labs,

(5) MLN4924 Inhibits Defective Ribosomal Product Antigen Presentation Independently of Direct NEDDylation of Protein AntigensThe Journal of ImmunologyMay 15, 2022Kartikeya Vijayasimha, Amy L. Leestemaker-Palmer, James S. Gibbs, Jonathan W. Yewdell, Brian P. Dolanpolyclonal antibodies were also used: anti-OVA (Sigma, C6534), anti beta-actin (Bethyl Laboratories, A300–485A, Lot# 3), anti-NUB1 (Cell Signaling, 14810S, Lot#1), and anti-NEDD8 (Cell Signaling 2745S, Lot#3). Infrared

(6) Human Respiratory Syncytial Virus NS2 Protein Induces Autophagy by Modulating Beclin1 Protein Stabilization and ISGylationmBioFebruary 22, 2022Chiok K, Pokharel SM, Mohanty I, Miller LG, Gao SJ, Haas AL, Tran KC, Teng MN, Bose S.no. 8025S) were purchased from Cell Signaling Technologies (MA, USA). Rabbit anti-actin (catalog no. A300-485A) was purchased from Bethyl Laboratories (TX, USA). Mouse anti-Flag (catalog no. F1804) and mouse anti-HA

(7) Topical Treatment of Human Skin and Cultured Keratinocytes with High-Dose Spironolactone Reduces XPB Expression and Induces ToxicityJID InnovSeptember 1, 2021Carpenter MA, Kemp MG.of antibodies against XPB (Santa Cruz sc-293, Santa Cruz Biotechnology, Dallas, TX), actin (Bethyl A300-485A, Bethyl Laboratories, Montgomery, TX), or phosphorylated Chk1 (Ser345; Cell Signaling #2348, Cell Signaling Technology

(8) Direct Conjugation of NEDD8 to the N-Terminus of a Model Protein Can Induce DegradationCellsApril 9, 2021Kartikeya Vijayasimha, Marilyn Vo Tran, Amy L. Leestemaker-Palmer, Brian P. Dolanrabbit polyclonal antibodies were also used: anti beta-actin (Bethyl Laboratories, Montgomery TX, USA, A300-485A, Lot#3), anti-NUB1 (Cell Signaling, Danvers MA, USA, 14810S, Lot#1), anti-ATG7 (Cell Signaling, D12B11,

(9) Influenza-Induced Oxidative Stress Sensitizes Lung Cells to Bacterial-Toxin-Mediated NecroptosisCell reportsAugust 25, 2020Gonzalez-Juarbe N, Riegler AN, Jureka AS, Gilley RP, Brand JD, Trombley JE, Scott NR, Platt MP, Dube PH, Petit CM, et al.Cell Signaling Technologies), -p-MLKL (1:1000, #37333S, Cell Signaling Technologies) and -cytoskeletal actin (1:10000, #A300–485A, Bethyl Laboratories Inc., Montgomery, TX), in 5% BSA overnight. Then, membranes were washed 3 times

(10) Lytic Cell Death Mechanisms in Human Respiratory Syncytial Virus-Infected Macrophages: Roles of Pyroptosis and NecroptosisVirusesAugust 25, 2020Bedient L, Pokharel SM, Chiok KR, Mohanty I, Beach SS, Miura TA, Bose S.# 9662). The actin antibody was purchased from Bethyl Laboratories, Montgomery, TX, USA (catalog # A300–485A). In some experiments, THP-1 cells were pre-treated with caspase-3 inhibitor Ac-DEVD-CHO (100 µM) for

(11) Epistatic Interplay between Type IV Secretion Effectors Engages the Small GTPase Rab2 in the Brucella Intracellular CyclemBioMarch 31, 2020Smith EP, Cotto-Rosario A, Borghesan E, Held K, Miller CN, Celli J.no. 15420-1-AP), and rabbit polyclonal anti-β-actin (1:20,000; Bethyl Laboratories, Inc.; catalog no. A300-485A). The following horseradish peroxidase (HRP)-conjugated secondary antibodies were used at 1:10,000 dilution:

(12) Integrin activation by the lipid molecule 25-hydroxycholesterol induces a proinflammatory responseNature communicationsApril 1, 2019Pokharel SM, Shil NK, Gc JB, Colburn ZT, Tsai SY, Segovia JA, Chang TH, Bandyopadhyay S, Natesan S, Jones JCR, et al.antibodies (Abcam, Cambridge, UK) were also used for western blot analyses. The actin antibody (catalog no. A300-485A) (1:5000) was purchased from Bethyl Laboratories (Texas, USA). In some experiments, protein bands from

(13) MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End JoiningMolecular cellJuly 19, 2018Hung PJ, Johnson B, Chen BR, Byrum AK, Bredemeyer AL, Yewdell WT, Johnson TE, Lee BJ, Deivasigamani S, Hindi I, et al.Armenian hamster anti-MRI,This paper,Clone 13E10.E12.C10 Rabbit anti-cytoskeletal actin,Bethyl Laboratories,Cat#A300-485A Mouse anti-FLAG,Sigma,Cat#F1804; clone M2 Rabbit anti-FLAG,Sigma,Cat#F7425 Rabbit anti-HA,Santa Cruz,Cat#sc-805 Mouse

(14) The Integrator complex controls the termination of transcription at diverse classes of gene targetsCell researchMarch 1, 2015Skaar JR, Ferris AL, Wu X, Saraf A, Khanna KK, Florens L, Washburn MP, Hughes SH, Pagano M.

(15) Anthocyanins protect human endothelial cells from mild hyperoxia damage through modulation of Nrf2 pathwayGenes & nutritionJuly 1, 2013Francesco Cimino, Antonio Speciale, Sirajudheen Anwar, Raffaella Canali, Elisabetta Ricciardi, Fabio Virgili, Domenico Trombetta, Antonina Saija

(16) Inactivation of max-interacting protein 1 induces renal cilia disassembly through reduction in levels of intraflagellar transport 20 in polycystic kidneyThe Journal of biological chemistryMarch 1, 2013Je Yeong Ko, Kyung Hyun Yoo, Seon Ah Song, Do Yeon Kim, Hyun Kyung Kong, Curie Ahn, Han Woong Lee, Duk-Hee Kang, Goo Taeg Oh, Jong Hoon Park

(17) Enteric-delivered rapamycin enhances resistance of aged mice to pneumococcal pneumonia through reduced cellular senescenceExperimental gerontologyDecember 1, 2012Hinojosa CA, Mgbemena V, Van Roekel S, Austad SN, Miller RA, Bose S, Orihuela CJ.

(18) CKI isoforms α and ε regulate Star-PAP target messages by controlling Star-PAP poly(A) polymerase activity and phosphoinositide stimulationNucleic acids researchOctober 1, 2011Laishram RS, Barlow CA, Anderson RA.

(19) The NIP7 protein is required for accurate pre-rRNA processing in human cellsNucleic Acids ResearchJanuary 1, 2011Luis G. Morello, Cédric Hesling, Patrícia P. Coltri, Beatriz A. Castilho, Ruth Rimokh, Nilson I. T. Zanchin

(20) GCN2 protein kinase is required to activate amino acid deprivation responses in mice treated with the anti-cancer agent L-asparaginaseThe Journal of biological chemistryNovember 20, 2009Piyawan Bunpo, Allison Dudley, Judy K Cundiff, Douglas R Cavener, Ronald C Wek, Tracy G Anthony

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Rabbit anti-Cytoskeletal Actin Antibody, Affinity Purified from Bethyl Laboratories, Inc.

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Bethyl Laboratories, Inc.

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Rabbit anti-Cytoskeletal Actin Antibody, Affinity Purified from Bethyl Laboratories, Inc.

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